[Histonet] When to use tap water?

[Gudrun Lang]

Hi
I think distilled water was formerly a rather expensive reagens in
laboratory. And so in each procedure, where Aqua dest. is not really
necessary, the technicians prefer to use tap water.
In my opinion tap water is necessary for blueing after hematoxylin and after
the Schiff reagens , and tap water is forbidden with all silverstains before
the fixation step. Otherwise there is no difference to rinse the slides with
tap or dest. water.

Gudrun Lang
general hospital, Linz, Austria

----- Original Message -----
From: <mrl0627 <@t> mail.ecu.edu>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, October 29, 2003 4:03 PM
Subject: [Histonet] When to use tap water?


>
> Hello, all:
> The prof for my graduate course in histology insisted that tap water be
used for rinsing slides during certain staining procedures (eg.
H&amp;E)although he did not give a specific reason why.
> When no &quot;flavor&quot; of water is specified in a procedure, I
generally use MilliQ or distilled deionized water.  Is this the safest route
to take or should one use tap if nothing but &quot;water&quot; is listed?
> Thanks for the attention.  Maureen, MS candidate at ECU.
>
> -----Original Message-----
> From: &quot;Houston, Ronnie&quot; &lt;Ronnie_Houston <@t> bshsi.com&gt;
> To: &quot;'Morken, Tim - Labvision'&quot; &lt;tpmorken <@t> labvision.com&gt;,
histonet <@t> lists.utsouthwestern.edu
> Sent: Tue, 28 Oct 2003 13:05:45 -0500
> Subject: RE: [Histonet] Dionized vs distilled water
>
> What quality of water is recommended/regulated for anatomic and clinical
> pathology labs?
> Ronnie Houston
> Regional Histology Operations Manager
> Bon Secours HealthPartners Laboratories
> 5801 Bremo Road
> Richmond, VA 23226
> (804) 287 7972
> ronnie_houston <@t> bshsi.com
> -----Original Message-----
> From: Morken, Tim - Labvision [mailto:tpmorken <@t> labvision.com]
> Sent: Tuesday, October 28, 2003 12:53 PM
> To: 'JCarpenter764 <@t> aol.com'; histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] Dionized vs distilled water
>
>
> Distilled water is classically produced by heating water to evaporation
> and subsequent condensing on a cold surface. In the process most
> impurities are either evaporated off ahead of the water (in the case of
> most organics), or left behind (in the case of minerals). The water is
> also effectively deionized because the salts are left behind. It is
> fairly pure water. To get very pure water it needs to be re-distilled
> several times.
>
> Deionized water is classically passed through a salt bed or ionized
> resin bed that captures the mineral ions (ie, a &quot;water
softener&quot;). The
> water is not necessarily pure, however, especially in regards to organic
> chemicals. Reverse osmosis is also used now days to deionize water.
>
>
> High quality water systems these days are some combination of filters,
> distillation, deionizing resins and reverse osmosis.
>
> Tim Morken
>
> -----Original Message-----
> From: JCarpenter764 <@t> aol.com [mailto:JCarpenter764 <@t> aol.com]
> Sent: Tuesday, October 28, 2003 7:45 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] (no subject)
>
> while studying for my exam on the different fixatives and there
> ingredients....i have noticed that some call for distilled water and
> some use the term deionized water.  Is there a difference?
>
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