[Histonet] RE: Microwave Tissue Processors

[Mary Reeves]

Amy,
Do you currently have a microwave tissue processor?  We use the Hacker/Milestone RHS-1 Microwave to process our fatty tissue. The times that you see only represent the time at temperature, they do not include the "ramp" up time (the time needed to bring the solution to the appropriate processing temperature).  

Fixation - We fix the tissue cassettes overnight (6pm - 3am) in Alcoholic  Zinc Formalin
Ethanol - 10 minutes at 65 C
JFC - Produced by Hacker. Isopropanol can be used instead, however, you may need to extend the time at temperature. - 1 hour and 37 minutes at 68 C 
Vaporization (Vacuum Drying) -Pressure at 500 mbar
Paraffin - 48 minutes at 65 C and pressure at 100 mbar

This program takes approximately 4 hours to run, it replaces our 16 hour overnight program that we previously used to process fatty tissue.  The only fatty tissue we do not process in the microwave is lipoma. 

Mary Reeves
Technical Specialist
Histology
1-352-265-0680 ext 7-2113

>>> Amy Self <ASelf <@t> gmhsc.com> 10/28/03 01:58PM >>>

	Mary,

	Could you share with me your procedure for processing fatty tissue
in the microwave.  

	Thanks,
	Amy Self

-----Original Message-----
From: Mary Reeves [mailto:REEVEML <@t> shands.ufl.edu] 
Sent: Tuesday, October 28, 2003 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu; thallada <@t> noch.org 
Subject: RE: [Histonet] RE: Microwave Tissue Processors


We microwave process all of our biopsy specimens and our fatty tissue
(except lipoma).   

Mary Reeves
Technical Specialist
Histology
1-352-265-0680 ext 7-2113

>>> "Hallada, Teri" <thallada <@t> noch.org> 10/28/03 11:42AM >>>
I was wondering if anyone out there is using a microwave tissue processor
for routine hospital tissues. Are there any regulations applicable to
instituting one, ie FDA approval?
Teri Hallada BS MT CT (ASCP)
thallada <@t> noch.org 
 



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