[Histonet] Help with cell culture

[david moses]

Dear Histonetters
I'd highly appreciate your opinion on following issue:
Neural cell cultures are fixed with 4% PFA and then permeabilized with 
Triton.Some people use brief exposure to Triton some incubate primary Ab for 
quite a long time with Triton.
Another approach is to fix/permeate cultures with methanol 20 min for -20.
I wondered about the impact of different methods on antigen detection - 
tubulin,NF,GFAP etc'.Of course,excluding oligodendrocyte stains.
Thanks
David Moses
Research Fellow

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