[Histonet] Re: Histonet digest, Vol 1 #103 - 18 msgs

[Amos Brooks]

Noelle,

         We've been working on this for a long time with constant 
inconsistant results. We've used many vendors antibodies and none have 
performed properly. (BTW, we run a multitude of other antibodies and they 
all work fine so the problem is the antibody not the rest of the process)
         If you're doomed to follow the VEGF path I highly advise that you 
run negative controls on it. You're bound to notice similarities. If you 
read about VEGF in literature do so with a highly skeptical mind.

Good luck,
Amos Brooks



At 01:00 PM 10/27/03, you wrote:
>Hi all!
>
>Does anyone have a decent protocol for VEGF, preferably on rat FFPE?  =
>
>Thank you!!
>
>Noelle Linke, BS, HTL(ASCP)
>Allergan, Inc
>2525 Dupont Drive RD-2A
>Irvine, CA 92612
>714-246-5568