[Histonet] pig neutrophil labeling

Sarah E Lamont slamont <@t> u.washington.edu
Wed Oct 22 15:35:33 CDT 2003


Has anyone had experience labeling neutrophils in pig skin?  I would
like to use an immunohistochemistry method to quantify the number of
neutrophils in the skin.

I'm using frozen sections, and have tried using rabbit anti-human
myeloperoxidase from Dako on PFA fixed and unfixed tissue with little
success.

Thanks in advance for any advice you could share!

Sarah Lamont
Research Scientist
Department of Bioengineering
University of Washington
Seattle, WA




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