[Histonet] fixative for insects

[Geoff McAuliffe]

A formalin - alcohol - acetic acid mix is often used for insects.There 
are many variations on the exact composition but Lillie (1976) recommends:
"concentrated formalin" ( 37-40% formaldehyde) :  10 ml
glacial acetic acid:  5ml
95% ethanol:  85 ml.
Fix for up to 24 hours.

Geoff

Andrea Grantham wrote:

> I'm going to be processing whole mosquitoes and I was wondering what 
> would be the best fixative to use...and processing schedule if anybody 
> has one.
>
> Thanks.
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
> : (office:  AHSC 4212)          P.O. Box 245044                     :
> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth <@t> u.arizona.edu)       :
> :...................................................................:
>           http://www.cba.arizona.edu/histology-lab.html
>
>
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-- 
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**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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