[Histonet] Mouse Embryo Processing

Tue Oct 14 17:38:34 CDT 2003

Stephanie

It may be the type of paraffin you are using. Surgipath makes a great
infiltration medium for the processor. It is called infiltration medium.
The embedding medium would be EM 400. I would also add some time to the
paraffin - 45 min. x3.

Something else you may want to think about is using a xylene substitute
instead of xylene.

Hope this helps.

Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot <@t> leica-microsystems.com
www.leica-microsystems.com

                      Stephanie Schmoll                                                                                                     
                      <sschmoll <@t> umich.edu>                To:       histonet <@t> lists.utsouthwestern.edu                                       
                      Sent by:                            cc:                                                                               
                      histonet-admin <@t> lists.utsouth        Subject:  [Histonet] Mouse Embryo Processing                                      
                      western.edu                                                                                                           

                      10/14/2003 03:53 PM                                                                                                   

I am fairly new to tissue processing with paraffin, have always done
cryo-embedding in the past.  I am having trouble processing late stage
mouse embryos, day 17 and 18.  The paraffin doesn't seem to be
infiltrating.  Any suggestions would be appreciated.

My current protocol is as follows:  24hrs 10% NBF, 70% EtOH 30 min, 80%
30 min, 95% 30 min x2, 100% 30 min x2, Xylene 30 min x2, paraffin 30
min x3.

Stephanie Schmoll, Morphology Core Manager
Center for Organogenesis
University of Michigan Medical School
5795C  Medical Science Bldg.  II
Phone: (734) 647-9021
Fax: (734) 647-9559

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