[Histonet] IHC QC's

[Patti Loykasek]

There was in fact, a post from Nick Kirk on running a positive control with
each case. I do realize the CAP requirements and am familiar with the
checklist. 

Patti Loykasek
Phenopath Laboratories
Seattle, WA
> 
> 
> I don't think anyone said a POSTIVE control should be run with each slide.
> We were talking about negative controls, I believe.
> I just copied and pasted from the latest CAP survey in another email.
>  
>  
> Hazel Horn, HT/HTL (ASCP)
> Histology Supervisor
> Arkansas Children's Hospital
> 
> Phone - 501.364.4240
> Fax - 501.364.3912
>> 
>> -----Original Message-----
>> From: Patti Loykasek [mailto:ploykasek <@t> phenopath.com]
>> Sent: Monday, October 13, 2003 10:52 AM
>> To: histonet
>> Subject: [Histonet] IHC QC's
>> 
>> I'm glad that everyone is so concerned with both negative and positive IHC
>> controls. There is certainly more than one side to this issue. I will say
>> that I don't think a positive QC on every slide is absolutely necessary, for
>> many reasons. If the QC is rare & precious, then it is a waste of resources.
>> As is running a negative control for every possible technique permutation on
>> small amounts of tumor. I would rather have slides with tumor left for
>> additional studies than have wasted tumor sections on 4-6 negative controls.
>> You can always evaluate non-specific staining on slides that have had an
>> antibody applied & that are negative with that antibody. The CAP is specific
>> that positive controls be used for each antibody - see  CAP checklist
>> ANP.22550. They do not specify for each slide. Since positive QC's should be
>> kept filed for the same number of years as the patient slide & records, it
>> should be possible to pull a QC slide from the IHC run for a particular
>> slide. In the CAP comment on ANP.22550, the use of internal QC's is also
>> mentioned. Although there are many ways of dealing with the issue of QC's,
>> I'm sure we all want to do what is prudent, abide by the regulations, and
>> increase the level of patient care.
>> Just my 2 cents worth.
>> 
>> Patti Loykasek
>> Phenopath Laboratories
>> Seattle, WA 
> 
> The information contained in this message may be privileged and confidential
> and protected from disclosure. If the reader of this message is not the
> intended recipient, or an employee or agent responsible for delivering this
> message to the intended recipient, you are hereby notified that any
> dissemination, distribution or copying of this communication is strictly
> prohibited. If you have received this communication in error, please notify us
> immediately by replying to the message and deleting it from your computer.
> Thank you. Arkansas Children's Hospital
> 


-------------- next part --------------
An HTML attachment was scrubbed...
URL: http://lists.utsouthwestern.edu/pipermail/histonet/attachments/20031013/c8745f80/attachment.htm