[Histonet] CD15 and CD3 more info

[Rena Fail]

Sorry I should have added both are at a dilution of 1:100, both are heat
retrieved with a buffer pH6.0 ,in a steamer for 20 minutes with a 10
minute cool down
Rena

-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of Robert
Fauck
Sent: Thursday, October 09, 2003 6:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD15 and CD3


Hi

Could any one suggest how to enhance the "crispiness" of CD3 and
CD15"staining"around the lymphocytes? We have some problem with both
DAKO and NOVOCASTRA for these two markers.

Thanks, Robert Fauck



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