[Histonet] Mouse brain

[Cheryl Crowder]

Annette - We have worked with a lot of mice brains and have had your 
problem.  We cut all the brains and CNS tissues at one time (not 
interspersed with other pieces and parts).  We use a waterbath at 37C.  
This helps the paraffin stay next to the tissue.  One of the problems 
with mice is that they can over-dehydrate easily and need shorter 
processing times.  When the tissue hits the water it "goes crazy" 
sucking up the water into the dehydrated tissues.  We also have 
moistened a slide and then placed the section directly on it, draining 
the excess water.  Try that if all else fails.  Hope this helps.  Cheryl


Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Anatomic Pathology
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Skip Bertman Drive
Baton Rouge, LA  70803

225-578-9734
FAX:  225-578-9720