[Histonet] Purple Haze.....

[Nick Kirk]

Roxanne

I can't see how the temperature of the water on the stainer could be causing
this artefact as the problem tends to be intermittent even when batch
staining.
Sometimes within a rack of slides you will get some slides from GI biopsies
showing the artefact while others from the same clinic session do not.
It has to be something individual to each specimen in my opinion, otherwise
we would see every slide going through on that particular batch exhibiting
the same staining artefact, which we don't.
I still think the actual biopsy process has a major part to play, especially
as that's the only variable we don't have any control over and all the
others we do.
The temperature thing is a spurious argument in my opinion when you consider
all blocks are chilled prior to cutting so everything is cold at some point
and if you do immuno antigen retrieval you apply excessive heat to the slide
and still get good nuclear detail when the section is counterstained.
I think it has to be something else.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England
  -----Original Message-----
  From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of
GREYTRUNK <@t> aol.com
  Sent: 09 October 2003 03:29
  To: ryaskovich <@t> dir.nidcr.nih.gov; STEGTM <@t> samcstl.org;
KMMerril <@t> LancasterGeneral.org; histonet <@t> pathology.swmed.edu
  Subject: Re: [Histonet] Purple Haze.....


  Check the temperature of the water on your stainer.
  Roxanne
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