[Histonet] negative IHC controls

[Sebree Linda A.]

L.Vermast,
 
We run a negative control with each patient slide/antibody.  In some cases we can use a negative control for more than one antibody on the same patient IF the antibodies are from the same source, ie rabbit, mouse, ascites fluid, and if the staining protocols are identical.   We also have statements from several of our pathologists that if their case has multiple blocks, one negative/antibody/CASE will suffice rather than one negative/antibody/BLOCK.
 
We've never run into a problem doing it this way.
 

	-----Original Message----- 
	From: vermast [mailto:vermast <@t> rogers.com] 
	Sent: Wed 10/8/2003 3:56 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] negative IHC controls
	
	
	 
	I would like to get a feel for how many out there are running negative control slides for IHC.  
	 
	In our lab we do just a handful of antibodies and initially I had been running a negative control slide with each patient slide.   After much discussion with our pathologists, we decided to omit these negatives (which were conistently negative) and continue to just run a positive control with each primary antibody for the run.  We use the Dako autostainer and prediluted primaries.  The decision to stop running negatives also coincided with Dako's decision to sell the negative control sera separately from the primaries (they used to come packaged together).  Perhaps I assumed that discontinuing to pair these reagents together meant that few labs were using the negatives.
	 
	Anyhow, after having reviewed the last QMPLS (Canada) survey committee comments, I believe the committe would like a negative control run with each patient tissue slide in order to evaluate background  (they have used NCCLS guide pages as reference).  Incidentally we weren't a part of the survey due to a technicality.
	Any help or advice would be appreciated.
	 
	L. Vermast
	Stratford, Ont.