[Histonet] Mouse tissue

Starkus, Laurie StarkusL <@t> ummhc.org
Wed Oct 8 10:29:38 CDT 2003


I've just started doing research with mouse tissue and had some difficulty
flattening the tissue in the water bath so that I got no folds in the
tissue.  I've addressed this issue by turning up the temperature on the
water bath.  However, now, I am getting a complaint that some of the tissues
are damaged.  Some of the tissue is disattached from the main tissue.  What
temperature do you all use your water baths at?  I'm using gelatin in the
water bath and coated slides.  Anyone have any advice on cutting mouse
tissue.  I've never had this problem with human tissue.




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