[Histonet] Bird feather

[T. Truscott]

Lalen, I would try rinsing your decal solution out of the tissue with water 
for about ten minutes to make sure all formic acid is gone. then procede as 
usual. Formic acid left in the tissue can interfere with adhesion. good luck 
Tom T. USDA Pullman WA.


>From: "Sarah Jones" <SJones <@t> cvm.tamu.edu>
>To: <lalsing <@t> cvdls.ucdavis.edu>,<histonet <@t> lists.utsouthwestern.edu>
>Subject: Re: [Histonet] Bird feather
>Date: Wed, 01 Oct 2003 23:20:54 -0500
>
>Have you tried celloidin coating the sections after deparaffinization?
>Are they lifting in the oven before deparaffinization?  I have the same
>trouble with horse hoof.  I use the ultrastick goldseal slides, but
>still have trouble from time to time.  You could try plastic embedding
>as a last resort.   Sarah
>
>Sarah Jones HT(ASCP)
>Dept. of Vet. Anatomy & Public Health
>Histology Lab
>Texas A&M University
>College Station, TX 77843-4458
>phone: 979-845-3177
>fax:  979-458-3499
>
>
> >>> "Elena Alsing" <lalsing <@t> cvdls.ucdavis.edu> 10/01/03 04:15PM >>>
>Greetings to all!
>How do you make bird feather stay on the slide? We received feather
>shafts in formalin. I decalcified them in equal amount of sodium citrate
>and formic acid and then process them. They cut nicely but they keep on
>lifting off the
>slide. I put them on plus slides and air dried them over the weekend -
>didn't work. I put them on probe-on slides, let them dry dry overnight
>at room temp and hand-stained them - didn't work. I don't know what to
>do... Please HELP!
>Thanks.
>Lalen Alsing
>UC Davis-CAHFS, Fresno
>
>
>
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