[Histonet] Cell Culture Staining

[Favara, Cynthia (NIH/NIAID)]

Why do you need xylene? they are not in paraffin?
c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Johnson, Kevin [mailto:KJohnson <@t> med.miami.edu]
Sent: Friday, October 03, 2003 3:29 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Cell Culture Staining


Greetings, all.

A researcher just brought me (at Miller Time on a Friday!) two culture
flasks of terminally differentiated Sertoli cells and requested H&E
staining.  Well.  

Conventional H&E obviously is out, since polystyrene is not compatible with
xylene.  Further restrictions: the cells cannot be scraped off, they cannot
be replated on glass coverslips, and for reasons of her own, they cannot go
back into the incubator pending an answer.  They now have been fixed in situ
with 10% neutral buffered formalin.

Does anyone have a protocol for staining these puppies?  If not H&E per se,
then is there another staining method that might satisfy her?

Thanks in advance, 

Kevin Johnson
University of Miami
Diabetes Research Institute


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