[Histonet] Aplha B crystalline and pathological conditions

[Delahaije P.]

Hi!
I'm trying to detect alpha B crystalline with an ELISA assay. I have two antibodies (mono and polyklonal) but i havent got a clue about wich buffers to use.
 
I would also like to know if anybody knows something about the pathological conditions alpha b crystalline is produced/overexpressed or used to determine a certain kind of disease? (for instance Alzheimer and so on....) 
 
thanks a lot!
 
Peter Delahaije,
Laboratory of pediatrics & neurology. 
Nijmegen Netherlands