[Histonet] DAKO ER and PR antibodies

[Patti Loykasek]

Just fyi. We have used Dako ER-1D5 at 1:200 & PR-636 at 1:250 using a
citrate pressure cook pretreatment and Envision+ detection.>I'm not sure
what could be giving you problems with your staining. How long is your
primary incubation? Detection incubation?

Patti Loykasek
PhenoPath Laboratories
Seattle, WA




 
> Try 1:10 for ER and 1:150 for PR with HIER pH 6.0 20 minutes steam, 10
> min cool down
> Rena Fail
> 
> -----Original Message-----
> From: histonet-admin <@t> lists.utsouthwestern.edu
> [mailto:histonet-admin <@t> lists.utsouthwestern.edu] On Behalf Of THERESA
> ROHR
> Sent: Thursday, October 02, 2003 11:00 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] DAKO ER and PR antibodies
> 
> 
> Hi out there in Histonet land:
> 
> I am having a problem working up dilutions for DAKO's ER (M7047) and PR
> (M3569) antibodies.
> I am using the Envision+ Detection system
> 
> Suggestions from DAKO were:
> 
> ER  1:100 to 1:150 with Envision+ and a 20 minute target retrieval PR
> 1:200 to 1:400 with Envision+ and a 20 minute target retrieval.
> 
> I've gone less and more and still cannot get any where near the staining
> on 
> these cases that went out to a IHC Laboratory than I am using as a
> comparison.
> 
> Any suggestions would certainly be appreciated.
> 
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