[Histonet] histogel

[Patsy Ruegg]

Has anyone ever tried using something like histogel to orient tissue for 
frozen sectioning?  I am wondering about the cutting and freezing quality of 
samples held in histogel til firm and then quick frozen in oct?  I need to 
get cross sections of mouse bile ducts.  I do this very successfully using 
histogel and paraffin processing, but I need to get frozen sections of the 
same for IHC T subset studies.
Please advise.
Best regards,
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg <@t> msn.com
wk email pruegg <@t> colobio.com

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