[Histonet] Superfrost Plus Slides

[Rita van Bendegem]

Dear All,

I have had some problems with Superfrosted slides but in our research lab we
process our IHC slides manual.  We react both frozens and paraffin tissues. Our
routine chromogens that we use are DAB, VIP, Alexa 488, and Alexa 594,
depending on the tissue and  the application.  We have been using Fisherbrand
Superfrosted Plus slides for years with no known problems.  In February of this
year I discovered that NF200 antibody from Sigma was not working at all in
frozen or paraffin tissue, but my positive control tissue was O.K. (since it was
cut on to older Superfrosted slides)
I pulled out some old paraffin slides dating back to Jan. 2002 and Sept. 2002
then ran the NF200 immuno.  The Jan. 2002 slides were positive, Sept. 2002 were
weakly positive and I also ran another Feb. 2003 slide which was negative.

This made me call my Fisher rep. about the problems and he in return called the
manufacture of the Superfrosted Plus slides, which from my understanding is ERIE
in North America. The Fisher rep. told me that he never got a response back from
the manufacture as of a few months ago.

In the meantime, I called a few techs  and made some inquires.  I was told to
try some slides from Surgipath that are called X-tra Adhesive they are European
slides with  USA, Canada, France and UK address written on the side of box.   I
have used them and have had no problems with my NF200 controls since.

I would be interested to hear if anyone else has had similar problems .




Bill Sinai wrote:

> Dear All,
>
> Has anyone had a problem with Superfrost Plus slides on automated
> Immunohistochemistry Staining machines?  If so what have the problems been?
> Also if the problem was resolved and how it was resolved.
>
> We obtain our slides through a third party agency in Australia.  We are at
> present using Menzel-Glaser (German) brand.  I am led to believe they are
> the largest slide and coverglass manufacturer in the world.
>
> Bill Sinai
> Laboratory Manager
> Tissue Pathology, ICPMR
> Westmead NSW 2145
> Australia
> Ph 02 9845 7774
>
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--
Rita van Bendegem, MLT
Laboratory Manager,
Canadian Paraplegic Association,
Spinal Cord Injury Research Centre,
Mc Laughlin Pavilion Lab 12-405,
399 Bathurst St.,
Toronto Western Hospital,
University Health Network,
Toronto, Ontario  M5T 2S8

Telephone 416-603-5800 ext. 2792
Fax 416-603-5745