[Histonet] Bird feather

[Sarah Jones]

Have you tried celloidin coating the sections after deparaffinization? 
Are they lifting in the oven before deparaffinization?  I have the same
trouble with horse hoof.  I use the ultrastick goldseal slides, but
still have trouble from time to time.  You could try plastic embedding
as a last resort.   Sarah

Sarah Jones HT(ASCP)
Dept. of Vet. Anatomy & Public Health
Histology Lab
Texas A&M University
College Station, TX 77843-4458
phone: 979-845-3177
fax:  979-458-3499


>>> "Elena Alsing" <lalsing <@t> cvdls.ucdavis.edu> 10/01/03 04:15PM >>>
Greetings to all!
How do you make bird feather stay on the slide? We received feather
shafts in formalin. I decalcified them in equal amount of sodium citrate
and formic acid and then process them. They cut nicely but they keep on
lifting off the
slide. I put them on plus slides and air dried them over the weekend -
didn't work. I put them on probe-on slides, let them dry dry overnight
at room temp and hand-stained them - didn't work. I don't know what to
do... Please HELP!
Thanks.
Lalen Alsing
UC Davis-CAHFS, Fresno



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet