[Histonet] Retic Stain

[Kelly Booher]

Barbara,

Is your Retic staining too light?  We leave our slides
in ammoniacal silver longer, and tone in gold chloride
for a shorter period of time.  Our procedure is as
follows:

0.5% Potassium Permanganate - 1 minute
Wash in tap water - 2 minutes
2% Potassium Metabisulfite - 1 minute
Wash in tap water - 2 minutes
2% Ferric Ammonium Sulfate - 3 minutes
Wash in tap water - 2 minutes
Rinse in deionized water
Ammonium Silver Nitrate - 3 minutes
Rinse in deionized water - 10 seconds
20% Formalin - 3 minutes
Wash in tap water - 3 minutes
0.2% Gold Chloride - 30 seconds to 1 minute
Rinse in deionized water
2% Potassium Metabisulfite - 1 minute
2% Sodium Thiosulfate - 1 minute
Wash in tap water - 2 minutes
Dehydrate, clear, and mount


Kelly Booher, BS, HTL (ASCP)
Anatomic Pathology
Swedish Medical Center, Providence Campus
Seattle, WA  98122

--- Barbara Murray <bamur <@t> alaska.net> wrote:
> Greetings,
> Our Retic stain need  to be improved. May I have
> some techniques on what everyone else is doing? We
> are leaving it in the Silver Solution  for 1 minute
> and the Gold Chloride for 5 mins. Everything else
> for 1 minute and washings for 2 minutes.
> Thanks in advance for all your suggestions.
> Have a great day!
> 
> Barbara. A. Murray, HT. (ASCP)
> The Alaska Native Medical Center
> Pathology Dept.
> Anchorage, Alaska
> 
> 


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