Thank you; Re: [Histonet] automated immunostainers

[Mauricio S. Morais]

Hello Histonetters.

I want to thank all that reply my original message.
They are all posted below for future reference if anyone else need it.

Thanks.

Mauricio S. Morais

Original Message:

Hello Histonetters.

Can anyone give me an idea about the costs of the machines (system) plus
monthly maintenance?
I wish to know if worth to have one to process 6-10 slides/day.

Thank you.

Mauricio S. Morais
Senior Research Technician
Nutrition, Exercise, Physiology, and Sarcopenia Laboratory (NEPS)
TUFTS University
Jean Mayer USDA Human Nutrition Research Center on Aging
711 Washington Street, Rm 436
Boston, MA 02111
(617) 556-3226

Replies:

1-
Mauricio, It may not seem that an instrument is necessary to process a
small
amount of slides, but you should ask yourself if having an uninterrupted
block of time is worthwhile. In otherwords, instead of tending to your
slides every few minutes for several hours, you could run an instrument
and
have that extended time to concentrate on something.

Lab Vision makes relatively inexpensive immunostaining instruments
(including a new 36-slide model) for $35K to $50K, but maybe that is not
what you need.

There are also some setups that make manual staining more efficient.
Look at
the ThermoShandon Sequenza immuno work station. 
http://www.thermo.com/eThermo/CDA/Products/Product_Detail/1,1075,10000056307
76-117-X-117-1000000008097,00.html

It allows you to easily add reagents without handling the slides. It is
faster than manual staining, but not expensive like an instrument. 


You might also look at the Biocare Medical Kinetic Slide Stainer.
http://www.biocare.net/equipment.htm
 
Again, it limits slide handling.

Good luck!

Tim Morken
Product Development
Lab Vision / NeoMarkers
47790 Westinghouse Dr
Fremont, CA 94539
PH: 510-991-2840
www.labvision.com

2-
Dear Mauricio,

Greetings! I happen to see this request on the Histonet and though of
responding to you directly. I represent Biocare Medical, which is
relatively
new company that offers Semi-Manual Kinetic Slide Stainer and other
related
Immunohistochemistry products. I am attaching a pdf literature on our IQ
Kinetic stainer that could hold 24-36 slides on the slide rack (12
slides on
each rack and comes in 2 or 3 racks). This slide holder eliminates the
slide
handling once loaded and has a Hot Bar that could be controlled
individually
between RT-99 Degree C (Programmable). For washes, the rack could be
place
in an inclined position on grooves thus eliminating slide handling. The
kinetic effect provided by the shaker helps better reaction at a lower
time.
The reagents are to be added manually.

Please read the prices on the 24 slides as $5795 in the literature. We
also
offer Polymer Detection and Double stain detection cocktails and kits.
Visit
our web site www.biocare.net for further details. If you any further
questions or concerns, please do not hesitate to contact me.

Best regards,

Hari Kumar
Technical Sales (Research Division)
Biocare Medical
Phone:    (925) 952-3901
Fax:      (925) 944-7888
hari <@t> biocare.net

Please visit us at www.biocare.net for the latest.

3-
Hi Mauricio,

I am the sales consultant for Cell Marque and have sent you a couple of
attachments that gives details on the cost per slide with Cell Marque's
products.  We do not have an automated immunostainer but all of our
products
work for manual customers or automated customers.  Please let me know if
you
have any questions or would like to receive a catalog from our company.

Sincerely,

Laura Hughes
Cell Marque Corporation
Technical Sales Consultant

4-
DAKO autostainers run about $45,000 to buy and $5000/year in a
maintenance
contract.  It holds 48 slides/run.  You can use your own reagents.

Ventana NeXes autostainers are about $20-30,000 to buy and $5000/year in
a
maintenance contract.  It holds 20 slides/run.  You have to use their
reagents.

Jan Shivers
Scientist, IHC Lab
U of MN Veterinary Diagnostic Lab



"Mauricio S. Morais" wrote:
> 
> Hello Histonetters.
> 
> Can anyone give me an idea about the costs of the machines (system) plus
> monthly maintenance?
> I wish to know if worth to have one to process 6-10 slides/day.
> 
> Thank you.
> 
> Mauricio S. Morais
> Senior Research Technician
> Nutrition, Exercise, Physiology, and Sarcopenia Laboratory (NEPS)
> TUFTS University
> Jean Mayer USDA Human Nutrition Research Center on Aging
> 711 Washington Street, Rm 436
> Boston, MA 02111
> (617) 556-3226
>
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