[Histonet] Kappa, Lambda staining???

[GUTIERREZ, JUAN]

That's just the nature of the beast.  Kappa and Lambda are known for excesive background staining.  My sugestion: since you already have the Benchmark, get the probes.  I ran the probes and the IHC together this summer and the difference in results were amazing.  There is no background and more cells staining with the probe.  
I'm currently trying to convince my manager to switch us to probes without any luck so far.  In the meantime we are using an Fc Receptor Blocker from INNOVEX Biosciences as an option on our program.  It helps, but we still get some background.
 
Juan C. Gutierrez HT(ASCP)
	-----Original Message----- 
	From: Jesus Ellin [mailto:Ellin13 <@t> adelphia.net] 
	Sent: Tue 9/30/2003 7:00 PM 
	To: histonet <@t> lists.utsouthwestern.edu 
	Cc: 
	Subject: [Histonet] Kappa, Lambda staining???
	
	
	Hello everyone ,, Need some help here, currently our lab is trying to create a IHC protocol for kappa and lambda on the Venntana benchmark,, we are having some real problems with the background staining in our control and patients,, we are using a blocker and or hydrogen peroxide, to try and minimize the peroxidase but we are still getting alot of background,  if any one out there that has a benchmark and has a protocol for these stains some help would be appreciated,, forgot to mention that we are using Tonsil and Bone Marrow for control,
	 
	One other thing how many out there are using the Tamtron System,, we are currently in the building phase and would like to ask some people out there questions concerning proccess, codes and end user training
	 
	 
	Jesus Ellin HTL ASCP    
	Yuma Regional Medical Center
	jellin <@t> yumaregional.org