[Histonet] Kappa, Lambda staining???

[Dana Settembre]

Jesus,
I don't know about the Ventana but I do advise you to try diluting your
antibodies.
Dana Settembre
University Hospital-UMDNJ, Newerk, NJ


>>> Jesus Ellin <Ellin13 <@t> adelphia.net> 9/30/2003 5:00:18 PM >>>
Hello everyone ,, Need some help here, currently our lab is trying to
create a IHC protocol for kappa and lambda on the Venntana benchmark,,
we are having some real problems with the background staining in our
control and patients,, we are using a blocker and or hydrogen peroxide,
to try and minimize the peroxidase but we are still getting alot of
background,  if any one out there that has a benchmark and has a
protocol for these stains some help would be appreciated,, forgot to
mention that we are using Tonsil and Bone Marrow for control,

One other thing how many out there are using the Tamtron System,, we
are currently in the building phase and would like to ask some people
out there questions concerning proccess, codes and end user training


Jesus Ellin HTL ASCP    
Yuma Regional Medical Center
jellin <@t> yumaregional.org