[Histonet] Fixations

[Anne-Sophie Martinez]

Hello,

Could anybody please help me. I would like to know what is the best
fixation for IHC experiments (4% PFA or Bouin?), classical electron
microscopy (I am planning 2.5% glutaraldehyde in sodium cacodylate buffer)
and immunogold in electron microscopy (I am planning 0.5% glutaraldehyde in
sodium cacodylate buffer)? Is LR-white the best resin for immunogold
staining?

Thank you very much for your help.

Anne-Sophie

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Dr Anne-Sophie Martinez
School of Biology
University of St. Andrews
Bute Medical Buildings
St. Andrews
Fife, KY16 9TS
UK.

Tel + 44 (0) 1334 463546
Fax + 44 (0) 1334 463600