[Histonet] Caspase-3, antigen retrieval

[Jocelyn Mora]

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Hi,
I am having trouble keeping human tissue on slides undergoing antigen 
retrieval during Caspase-3 immunohistochemistry.
The antigen retrieval involves boiling the slides in a 0.1M Tris-HCl 
buffer for 20mins in the microwave, which is causing the tissue to 
fall off the slides! I have tried wrapping slides with gauze, and 
using different coated slides, but so far no luck.
I am looking for a different method of antigen retrieval other than 
the above, or treating the tissue with trypsin which is too harsh. 
Any suggestions would be most welcome!!
Thank-you,
Jocelyn Mora