[Histonet] fixing bone marrow for IHC

Tue Nov 25 15:56:06 CST 2003

We use formalin.  I hate to see anyone use B5 because of environmental
concerns with mercury.

Richard Cartun
Director, Immunopathology
Hartford Hospital

>>> Sharon Cooperman <scoop <@t> mail.nih.gov> 11/25/03 04:16PM >>>
Does anyone have an opinion on the best way to fix bone marrow smears 
for immunohistochemistry?  So far I've heard methanol, acetone, B-5 
and formalin.  The antibodies I plan to use all work on formalin 
fixed paraffin embedded tissue.  I'd appreciate any advice or 
opinions.

Thanks,
Sharon

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