[Histonet] In situ hybridization

[Carlene L. Zindl]

Hello,

Does anyone have experience with non-radioactive in situ hybridization of
frozen mouse tissue??  If so, I would appreciate advice on details such
as: 1) is biotin or dig better? 2) what is the best way to fix this
tissue?  3) what is the optimal size RNA probe?

Thank you for any pertinent information.
Sincerely,
Carlene Zindl
University of Alabama at Birmingham
clzindl <@t> artsci.wustl.edu -OR-  clzindl <@t> uab.edu