[Histonet] separated cells continued

[Tony Henwood]

Where is the xylene or other wax & ethanol miscible solvent?

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

http://www.histosearch.com/homepages/TonyHenwood/default.html
http://us.geocities.com/tonyhenwoodau/index.html



-----Original Message-----
From: Bernadette Weston [mailto:bernaweston <@t> hotmail.com]
Sent: Saturday, 22 November 2003 6:28 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] separated cells continued


Hope this helps my problem:
Here is our processing program:

Formalin  1:00
Formalin  2:30
70% ETOH   :50
80% ETOH   :50
95% ETOH   :50
95% ETOH   :50
100% ETOH :50
100% ETOH :50
Paraffin 1:00
Paraffin 1:00
Paraffin 1:00

The paraffin station are at 58 degrees the other stations are ambient the 
pressure is on all stations.

The sections do not appear to be improperly processed when cutting, we use 
accuride blades, we get a nice ribbon and the sections lay out in the 
waterbath, we cut at 3 and 4 microns.  We put the slides in a 60 degree 
oven.
pictures @ histonet images

Bernadette Weston HT

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