[Histonet] Biotin block after primary?

Favara, Cynthia (NIH/NIAID) cfavara <@t> niaid.nih.gov
Fri Nov 21 08:50:16 CST 2003


You betcha!
c
 

Cynthia Favara 
NIAID/NIH/RML/LPVD 
903 South 4th Street 
Hamilton, MT 59840 
406-363-9317 

-----Original Message-----
From: brade, brade upland, upland [mailto:newhistotech <@t> yahoo.com]
Sent: Wednesday, November 19, 2003 9:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Biotin block after primary?


Hi,
I accidently added my primary antibody before avidin/biotin blocking.  Is it
possible to block after the primary but before a biontinylated secondary?
Thank you for help.



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