[Histonet] Re: Histonet digest, Vol 1 #133 - 27 msgs
Cindy DuBois
ccdub <@t> earthlink.net
Wed Nov 19 17:11:12 CST 2003
There are 3 histolabs around my area and all of us start no later than 4
a.m.
on 11/17/03 10:00 AM, histonet-request <@t> lists.utsouthwestern.edu at
histonet-request <@t> lists.utsouthwestern.edu wrote:
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>
> Today's Topics:
>
> 1. what is histology (Michael F.)
> 2. re what is histology? (Carl)
> 3. RE: Perfusion pump time (Charles W. Scouten, Ph.D.)
> 4. RE: e-cadherin antibody (Scott Mordue)
> 5. Re: e-cadherin antibody (SAULSAULX <@t> aol.com)
> 6. Re: Decreased antigenicity with alcohol-formalin fixation (anita dudley)
> 7. Re: e-cadherin antibody (SAULSAULX <@t> aol.com)
> 8. Trichromes (was Re:What is Histology?) (John Kiernan)
> 9. cytokeratin (Lombaert)
> 10. Re: Exhaust Block (louise renton)
> 11. RE: thick frozen sections (Alan Bright)
> 12. Re: Trichromes (was Re:What is Histology?) (SAULSAULX <@t> aol.com)
> 13. Re: Trichromes (was Re:What is Histology?) (rfail <@t> toolkitmail.com)
> 14. Fwd: Re: [Histonet] Trichromes (was Re:What is Histology?) (Ian
> Montgomery)
> 15. Re: cytokeratin (Dennis Najarian)
> 16. Re: Trichromes (was Re:What is Histology?) (Bryan Hewlett)
> 17. Hansson's Carbnic Anhydrase. (leanneharris <@t> eircom.net)
> 18. What is histology? (Dawson, Glen)
> 19. RE: Anti-beta-galactosidase antibodies (Favara, Cynthia (NIH/NIAID))
> 20. RE: Hansson's Carbnic Anhydrase. (Houston, Ronnie)
> 21. polymerization failure _ Technovit 9100New (Tom Schaer)
> 22. Re: Re: [Histonet] Trichromes (Hernan Aldana Marcos)
> 23. Trichromes and "ten-page double-spaced paper" (Alex Knisely)
> 24. trichrome paper (Mary Lou)
> 25. Re: Trichromes (was Re:What is Histology?) (Gayle Callis)
> 26. Re: CSAII kit (Dana Settembre)
> 27. RE: re what is histology? (Chung, Luong)
>
> --__--__--
>
> Message: 1
> To: histonet <@t> lists.utsouthwestern.edu
> From: "Michael F." <le.paix <@t> verizon.net>
> Organization: Waikiki Coffee Inc.
> Date: Sun, 16 Nov 2003 09:31:58 -1000
> Subject: [Histonet] what is histology
>
> as an aside, could you folks tell what percent would you say of HT/HTL
> start work before 5am?
>
> would you say that an HT/HTL would be more likely to have opportunities to
> work in research than a MT/MTL?
>
> I'm considering one or the other schools. Hope you don't mind my off topic
> posting. Please email me directly if its more appropriate.
>
> cheers,
>
> M.
>
> ...back to lurking
>
>
> --__--__--
>
> Message: 2
> From: "Carl" <carl.hobbs <@t> kcl.ac.uk>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Date: Sun, 16 Nov 2003 19:52:55 -0000
> Subject: [Histonet] re what is histology?
>
> After a friend asked what I am/did, I expained about Histology, staining,
> dyes etc. I subsequently overheard him saying to another person that I was
> an artist! To him dyes/stains= paints...( hadn't listened to a word....or my
> explanation was inadequate lol)
> I suppose it's true; a picture paints a thousand words. Perhaps that's why,
> for example, the pathologist uses many words to describe what's seen in a
> section ;-)
>
>
>
> --__--__--
>
> Message: 3
> Date: Sun, 16 Nov 2003 17:20:17 -0600
> From: "Charles W. Scouten, Ph.D." <cwscouten <@t> myneurolab.com>
> To: "Davis, Gareth" <gareth.davis <@t> Vanderbilt.Edu>,
> "Histonet" <Histonet <@t> lists.utsouthwestern.edu>
> Subject: RE: [Histonet] Perfusion pump time
>
> This is a multi-part message in MIME format.
>
> ------_=_NextPart_001_01C3AC98.32688D72
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>
> I have responded separately with an attached 7 page discussion of
> perfusion, and a link to our Perfusion One product. Don't use flow
> rate, pressure is what the cardiovascular system controls, use 5%
> sucrose prewash, no saline, and switch to fixative when the animal
> stops. Haparin is not needed, all blood can be cleared without it with
> sufficient pressure.
>
> =20
>
> If any body else would like to see these materials, I will send them.
>
> =20
>
> Charles W. Scouten, Ph.D.=20
> myNeuroLab.com=20
> 5918 Evergreen Blvd.=20
> St. Louis, MO 63134=20
> Ph: 314 522 0300 =20
> FAX 314 522 0377=20
> cwscouten <@t> myneurolab.com <mailto:cwscouten <@t> myneruolab.com> =20
> www.myneurolab.com=20
>
> =20
>
> -----Original Message-----
> From: Davis, Gareth [mailto:gareth.davis <@t> Vanderbilt.Edu]=20
> Sent: Saturday, November 15, 2003 3:29 PM
> To: Histonet
> Subject: [Histonet] Perfusion pump time
>
> =20
>
> Hello Histonetters,
>
> Recently (within the last couple months) I saw someone post perfusion
> times for pumps based on the animal being perfused. I tried to find the
> posting on the Histosearch, but unsuccessful. We are perfusing rats for
> the olfactory tissue, but would like ideals on the what others have
> done. Any input would be appreciated, including if heparin is needed in
> saline, the correct perfusion pump time, and if recirculation is
> necessary.
>
> thanks,
>
> Gareth
>
> =20
>
> Ms. Gareth B. Davis
>
> Research Assistant II
>
> =20
>
> =20
>
>
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>
> <p class=3DMsoNormal><font size=3D3 color=3Dnavy face=3D"Times New =
> Roman"><span
> style=3D'font-size:12.0pt;color:navy'>I have responded separately with =
> an attached
> 7 page discussion of perfusion, and a link to our Perfusion One
> product. Don’t use flow rate, pressure is what the =
> cardiovascular
> system controls, use 5% sucrose prewash, no saline, and switch to =
> fixative
> when the animal stops. Haparin is not needed, all blood can be =
> cleared without
> it with sufficient pressure.</span></font></p>
>
> <p class=3DMsoNormal><font size=3D3 color=3Dnavy face=3D"Times New =
> Roman"><span
> style=3D'font-size:12.0pt;color:navy'> </span></font></p>
>
> <p class=3DMsoNormal><font size=3D3 color=3Dnavy face=3D"Times New =
> Roman"><span
> style=3D'font-size:12.0pt;color:navy'>If any body else would like to see =
> these
> materials, I will send them.</span></font></p>
>
> <p class=3DMsoNormal><font size=3D3 color=3Dnavy face=3D"Times New =
> Roman"><span
> style=3D'font-size:12.0pt;color:navy'> </span></font></p>
>
> <div>
>
> <p><font size=3D3 color=3Dnavy face=3D"Comic Sans MS"><span =
> style=3D'font-size:12.0pt;
> font-family:"Comic Sans MS";color:navy'>Charles W. Scouten, =
> Ph.D.</span></font><font
> color=3Dnavy><span style=3D'color:navy'> <br>
> </span></font><b><font color=3D"#339966"><span =
> style=3D'color:#339966;font-weight:
> bold'>myNeuroLab.com</span></font></b><font color=3Dnavy><span =
> style=3D'color:navy'>
> <br>
> </span></font><font size=3D2 color=3Dnavy face=3D"Bookman Old =
> Style"><span
> style=3D'font-size:10.0pt;font-family:"Bookman Old =
> Style";color:navy'>5918
> Evergreen Blvd.</span></font><font color=3Dnavy><span =
> style=3D'color:navy'> <br>
> </span></font><font size=3D2 color=3Dnavy face=3D"Bookman Old =
> Style"><span
> style=3D'font-size:10.0pt;font-family:"Bookman Old =
> Style";color:navy'>St. Louis,
> MO 63134</span></font><font color=3Dnavy><span style=3D'color:navy'> =
> <br>
> </span></font><font size=3D2 color=3Dnavy face=3D"Bookman Old =
> Style"><span
> style=3D'font-size:10.0pt;font-family:"Bookman Old =
> Style";color:navy'>Ph: 314 522
> 0300 </span></font><font color=3Dnavy><span =
> style=3D'color:navy'><br>
> </span></font><font size=3D2 color=3Dnavy face=3D"Bookman Old =
> Style"><span
> style=3D'font-size:10.0pt;font-family:"Bookman Old =
> Style";color:navy'>FAX
> 314 522 0377</span></font><font color=3Dnavy><span style=3D'color:navy'> =
> <br>
> <a href=3D"mailto:cwscouten <@t> myneruolab.com">cwscouten <@t> myneurolab.com</a> =
> <br>
> www.myneurolab.com </span></font></p>
>
> </div>
>
> <p class=3DMsoNormal><font size=3D3 color=3Dnavy face=3D"Times New =
> Roman"><span
> style=3D'font-size:12.0pt;color:navy'> </span></font></p>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D2 =
> face=3DTahoma><span
> style=3D'font-size:10.0pt;font-family:Tahoma'>-----Original =
> Message-----<br>
> <b><span style=3D'font-weight:bold'>From:</span></b> Davis, Gareth
> [mailto:gareth.davis <@t> Vanderbilt.Edu] <br>
> <b><span style=3D'font-weight:bold'>Sent:</span></b> Saturday, November =
> 15, 2003
> 3:29 PM<br>
> <b><span style=3D'font-weight:bold'>To:</span></b> Histonet<br>
> <b><span style=3D'font-weight:bold'>Subject:</span></b> [Histonet] =
> Perfusion pump
> time</span></font></p>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'> </span></font></p>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'>Hello Histonetters,</span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'>Recently (within the last couple months) I =
> saw someone
> post perfusion times for pumps based on the animal being =
> perfused. I
> tried to find the posting on the Histosearch, but unsuccessful. We =
> are
> perfusing rats for the olfactory tissue, but would like ideals on the =
> what
> others have done. Any input would be appreciated, including if =
> heparin is
> needed in saline, the correct perfusion pump time, and if
> recirculation is necessary.</span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'>thanks,</span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'>Gareth</span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'> </span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D2 =
> color=3Dpurple
> face=3DSystem><span =
> style=3D'font-size:10.0pt;font-family:System;color:purple'>Ms.
> Gareth B. Davis</span></font></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><strong><b><font =
> size=3D2
> color=3Dpurple face=3DSystem><span =
> style=3D'font-size:10.0pt;font-family:System;
> color:purple'>Research Assistant II</span></font></b></strong></p>
>
> </div>
>
> <div>
>
> <p class=3DMsoNormal style=3D'margin-left:.5in'><font size=3D3 =
> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'> </span></font></p>
>
> </div>
>
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>
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> face=3D"Times New Roman"><span
> style=3D'font-size:12.0pt'> </span></font></p>
>
> </div>
>
> </div>
>
> </body>
>
> </html>
> =00
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>
> --__--__--
>
> Message: 4
> Date: Sun, 16 Nov 2003 18:03:16 -0800 (PST)
> From: Scott Mordue <i_stain <@t> yahoo.com>
> To: alexander.nader <@t> wgkk.sozvers.at
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Subject: RE: [Histonet] e-cadherin antibody
>
> We are using monoclonal E-Cadherin in breast cancer
> tissues, clone: 4A2C7, from Zymed.
>
> Scott
> CSU
>
>
> From: "Nader, Alexander"
> <alexander.nader <@t> wgkk.sozvers.at>
> To: "'histonet-admin <@t> lists.utsouthwestern.edu'"
> <histonet-admin <@t> lists.utsouthwestern.edu>
> Cc: "'Histonet <@t> lists.utsouthwestern.edu'"
> <Histonet <@t> lists.utsouthwestern.edu>
> Date: Wed, 12 Nov 2003 08:07:01 +0100
> Subject: RE: [Histonet] e-cadherin antibody
>
>> Which clone are people using for e-caherin IHC on
> FFPE tissues?
>> Novocastra E-Cadherin clone 36B5 (IgG1) 1:50.
>
> Alexander Nader
> Inst. f. Pathology, Hanusch-Hospital
> Vienna, Austria
>
> __________________________________
> Do you Yahoo!?
> Protect your identity with Yahoo! Mail AddressGuard
> http://antispam.yahoo.com/whatsnewfree
>
>
> --__--__--
>
> Message: 5
> From: SAULSAULX <@t> aol.com
> Date: Sun, 16 Nov 2003 21:12:34 EST
> To: i_stain <@t> yahoo.com, alexander.nader <@t> wgkk.sozvers.at
> CC: Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] e-cadherin antibody
>
>
> --part1_1a5.1c1895a5.2ce98892_boundary
> Content-Type: text/plain; charset="US-ASCII"
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>
> Histotechies......! I need help.where can i get a ten page double space
> research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks
>
> --part1_1a5.1c1895a5.2ce98892_boundary
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> Content-Transfer-Encoding: quoted-printable
>
> <HTML><FONT FACE=3Darial,helvetica><FONT SIZE=3D2 FAMILY=3D"SANSSERIF" FACE=
> =3D"Arial" LANG=3D"0"><B><I>Histotechies......! I need help.where can i
> get=20=
> a ten page double space research paper on the following two stains:<BR>
> <BR>
> 1.Alcian Blue<BR>
> <BR>
> 2.Trichrome stain<BR>
> <BR>
> <BR>
> thanks</B></I></FONT></HTML>
>
> --part1_1a5.1c1895a5.2ce98892_boundary--
>
>
> --__--__--
>
> Message: 6
> From: "anita dudley" <azdudley <@t> hotmail.com>
> To: GoodwinD <@t> pahosp.com, HistoNet <@t> Pathology.swmed.edu
> Date: Mon, 17 Nov 2003 02:13:03 +0000
> Subject: Re: [Histonet] Decreased antigenicity with alcohol-formalin fixation
>
> diana, I use penfix on my processor and we have good luck with our ers and
> prs, we also use it sometime to fix breast tissue before going on the
> processor. haven't had any problems with it. hope this helps.
> anita dudley
> providence hosp
> mobile alabama
>
>
>> From: "Goodwin, Diana" <GoodwinD <@t> pahosp.com>
>> To: "Histonet (E-mail)" <HistoNet <@t> Pathology.swmed.edu>
>> Subject: [Histonet] Decreased antigenicity with alcohol-formalin fixation
>> Date: Fri, 14 Nov 2003 11:39:21 -0500
>>
>>
>>> Greetings, Histonetters.
>>>
>>> One of my pathologists has expressed concern that antigenicity for
>> certain
>>> antibodies, specifically ER and PR, in breast tissue that is processed
>>> using alcohol-formalin will be decreased. Can anyone cite a reference
>> in
>>> the literature that confirms or addresses this concern? Has anyone had
>>> this experience, or does anyone know of any reason that using
>>> alcohol-formalin (specifically Pen-Fix) would adversely affect IHC
>>> staining?
>>>
>>> Thanks to all of you,
>>>
>>> Diana Goodwin
>>> Supervisor, Anatomic Pathology
>>> Preston 6
>>> 215-829-6532
>>> e-mail: goodwind <@t> pahosp.com
>>>
>
> _________________________________________________________________
> Frustrated with dial-up? Get high-speed for as low as $26.95.
> https://broadband.msn.com (Prices may vary by service area.)
>
>
>
> --__--__--
>
> Message: 7
> From: SAULSAULX <@t> aol.com
> Date: Sun, 16 Nov 2003 21:15:04 EST
> To: SAULSAULX <@t> aol.com, i_stain <@t> yahoo.com, alexander.nader <@t> wgkk.sozvers.at
> CC: Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] e-cadherin antibody
>
>
> --part1_28.40452994.2ce98928_boundary
> Content-Type: text/plain; charset="US-ASCII"
> Content-Transfer-Encoding: 7bit
>
> In a message dated 11/16/2003 9:14:01 PM Eastern Standard Time,
> SAULSAULX <@t> aol.com writes:
>
>> Histotechies......! I need help.where can i get a ten page double space
>> research paper on the following two stains:
>>
>> 1.Alcian Blue
>>
>> 2.Trichrome stain
>>
>>
>> thanks
>
> Histotechies......! I need help.where can i get a ten page double space
> research paper on the following two stains:
>
> 1.Alcian Blue
>
> 2.Trichrome stain
>
>
> thanks
>
> --part1_28.40452994.2ce98928_boundary
> Content-Type: text/html; charset="US-ASCII"
> Content-Transfer-Encoding: quoted-printable
>
> <HTML><FONT FACE=3Darial,helvetica><FONT SIZE=3D2 FAMILY=3D"SANSSERIF" FACE=
> =3D"Arial" LANG=3D"0">In a message dated 11/16/2003 9:14:01 PM Eastern Stand=
> ard Time, SAULSAULX <@t> aol.com writes:<BR>
> <BR>
> <BLOCKQUOTE TYPE=3DCITE style=3D"BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT=
> : 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px"><B><I>Histotechies......! I nee=
> d help.where can i get a ten page double space research paper on the followi=
> ng two stains:<BR>
> <BR>
> 1.Alcian Blue<BR>
> <BR>
> 2.Trichrome stain<BR>
> <BR>
> <BR>
> thanks</FONT><FONT COLOR=3D"#000000" style=3D"BACKGROUND-COLOR: #ffffff" SI=
> ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0"></B></I> </BLOCKQUOTE>=
> <BR>
> </FONT><FONT COLOR=3D"#000000" style=3D"BACKGROUND-COLOR: #ffffff" SIZE=3D2=
> FAMILY=3D"SANSSERIF" FACE=3D"Arial" LANG=3D"0"><BR>
> <B><I>Histotechies......! I need help.where can i get a ten page double spac=
> e research paper on the following two stains:<BR>
> <BR>
> 1.Alcian Blue<BR>
> <BR>
> 2.Trichrome stain<BR>
> <BR>
> <BR>
> thanks</FONT><FONT COLOR=3D"#000000" style=3D"BACKGROUND-COLOR: #ffffff" SI=
> ZE=3D3 FAMILY=3D"SANSSERIF" FACE=3D"arial" LANG=3D"0"></B></I> <BR>
> </FONT></HTML>
> --part1_28.40452994.2ce98928_boundary--
>
>
> --__--__--
>
> Message: 8
> Date: Mon, 17 Nov 2003 00:47:47 -0500
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Reply-To: jkiernan <@t> uwo.ca
> To: SAULSAULX <@t> aol.com
> CC: Jackie.O'Connor <@t> abbott.com, tigersnake <@t> ecybermind.net,
> histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Trichromes (was Re:What is Histology?)
>
> The last 2 paragraphs of this email are not
> about trichrome staining.
>
> Are you (SAULSAULX <@t> aol.com) looking for a
> review of postulated mechanisms of differential
> staining in the various trichrome methods, or
> for 10 pages of practical instructions, or
> for comparisons of different trichrome methods?
> If you let us know what you need you (and
> other Histonetters) will probably get several
> reading lists!
>
> The staining mechanisms are controversial
> because there has been too much speculation
> and not enough research. In this field you'll
> need to read 3 or 4 review articles to try
> to form a balanced view of your own. The
> mechanisms for trichromes (methods using 3
> anionic dyes and PTA or PMA) need to be
> considered alongside methods using 2
> anionic dyes in one solution (notably
> Van Gieson's stain and its less often
> used congeners).
>
> Is your name really SAULSAULX? It's much
> more satisfying to reply to a name at a
> real location such as, for example,
> Abey C. Defghi at the Royal Spithouse Infirmary
> in Badchester, Rayneshire, England.
> Histonet is a friendly community of over
> 1000 (I think; Herb or Linda might correct
> the number), and most of us indicate who we are
> when we ask or answer questions.
>
> On this note (and nothing to do with trichrome
> stains), I'd like to say how happy I was to
> see many Histonetters face to face at the
> recent NSH meeting in Louisville. I wish I
> could have talked with more of you, but it's
> easy to miss people in a big crowd. Also, the
> names on the lapel badges were printed too
> small. Such badges should be readable at a
> distance. Though not a shy person, I did not
> want to stare closely at the name label on
> every comely bosom. In these litiginous times
> everyone must avoid being greatly misunderstood.
> (NSH label makers please take note! Not many
> people have telescopic vision. Our instrument
> is the microscope.)
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