[Histonet] Anti-beta-galactosidase antibodies

[Sharon Cooperman]

No, sorry.  Just out of curiousity, why do you want to do both the 
enzymatic and IHC?  Also, I think that if the enzyme isn't  destroyed 
by the reaction it might work - eg., I wouldn't expect it to work on 
peroxidase, but I don't know if beta gal activity persists after the 
reaction.

Sharon

>Sharon,
>Have you used this antibody on previously X-gal reacted tissue?  We 
>are using 40-1a.  Thanks, Maureen Loomer, MS candidate at ECU
>
>-----Original Message-----
>From: Sharon Cooperman &lt;scoop <@t> mail.nih.gov&gt;
>To: t-kuzniar <@t> md.northwestern.edu
>Sent: Tue, 18 Nov 2003 18:34:04 -0500
>Subject: Re: [Histonet] Anti-beta-galactosidase antibodies
>
>Hi.  Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed
>paraffin embedded tissue.  We used it on a transgenic mouse
>expressing Beta gal - pretty weak but detectable with ABC kit and
>HRP.  I think most of the transgenics express weakly and I would
>consider using tyramide (TSA) if you need more signal because
>background is very low.  I also think some of the Abcam anti-beta gal
>abs are pretty good (I don't know which ones, I borrowed them).
>
>Sharon
>
>&gt;Has anyone had any experience with polyclonal antibody against
>&gt;b-galactosidase? What reporter system have you used? Thank you.
>&gt;Tom Kuzniar
>&gt;
>&gt;
>&gt;
>&gt;_______________________________________________
>&gt;Histonet mailing list
>&gt;Histonet <@t> lists.utsouthwestern.edu
>&gt;http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>--
>Sharon Cooperman      	     &lt;scoop <@t> mail.nih.gov&gt;
>NIH, NICHD, CBMB                     301.435-7735
>Building 18T, room 101               301.402-0078 fax
>Bethesda, MD 20892
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>


-- 
Sharon Cooperman       	     <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892