[Histonet] Anti-beta-galactosidase antibodies

[mrl0627 <@t> mail.ecu.edu]

Sharon,
Have you used this antibody on previously X-gal reacted tissue?  We are using 40-1a.  Thanks, Maureen Loomer, MS candidate at ECU

-----Original Message-----
From: Sharon Cooperman &lt;scoop <@t> mail.nih.gov&gt;
To: t-kuzniar <@t> md.northwestern.edu
Sent: Tue, 18 Nov 2003 18:34:04 -0500
Subject: Re: [Histonet] Anti-beta-galactosidase antibodies

Hi.  Chemicon AB1211, ABC kit, HRP with DAB on formalin fixed 
paraffin embedded tissue.  We used it on a transgenic mouse 
expressing Beta gal - pretty weak but detectable with ABC kit and 
HRP.  I think most of the transgenics express weakly and I would 
consider using tyramide (TSA) if you need more signal because 
background is very low.  I also think some of the Abcam anti-beta gal 
abs are pretty good (I don't know which ones, I borrowed them).

Sharon

&gt;Has anyone had any experience with polyclonal antibody against
&gt;b-galactosidase? What reporter system have you used? Thank you.
&gt;Tom Kuzniar
&gt;
&gt;
&gt;
&gt;_______________________________________________
&gt;Histonet mailing list
&gt;Histonet <@t> lists.utsouthwestern.edu
&gt;http://lists.utsouthwestern.edu/mailman/listinfo/histonet


-- 
Sharon Cooperman       	     &lt;scoop <@t> mail.nih.gov&gt;
NIH, NICHD, CBMB                     301.435-7735
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet