[Histonet] Golgi-Cox Method

[John Kiernan]

The variant of Gibb & Kolb (1998) J. Neurosci. Methods
79:1-4 gives excellent impregnation of dendrites and
their spines. The use of a vibrating microtome to cut
the thick sections is an improvement over the
traditional nitrocellulose embedding or using frozen
sections.

With any Golgi-Cox procedure you'll be using large
amounts of mercuric chloride, so be careful. The used
solution cannot be sent down the drain.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan <@t> uwo.ca
   http://publish.uwo.ca/~jkiernan/
______________________________________

Sw918890 <@t> aol.com wrote:
> 
> I was wondering if anyone out there had a staining protocol for Golgi-Cox.  My principle investigator is having me investigate kits for this stain.  We have found a source, but I also want to see other protocols out there as well.
> 
> Thanks in advance,
> 
> Steven R. Westra
> University of Iowa Health Care
> (319) 356-1090
> 
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