[Histonet] Perfusions of young mice

[Favara, Cynthia (NIH/NIAID)]

Randa,

I have done as young as P5. I agree they are very small. Things I found most
helpful are:

	1. good lighting some type of fiber optic is what I use
	2. magnifying glasses - the kind that surgeons use some even have a
light source
	3. winged infusion needles 27Gx1/2"

I used 3-5 ml syringes just found it easier

and 

practice!practice!practice!

good luck!
	
Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317


-----Original Message-----
From: Randa Perkins [mailto:rperkinsfsu <@t> hotmail.com]
Sent: Monday, November 17, 2003 2:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Perfusions of young mice


To all:
  If anyone has any advice on perfusing very young mice I would be grateful 
to hear it.  We are trying to harvest the brains of mice at P9 (post-natal 
day 9) and younger.  I've been able to perfuse adult mice with the standard 
method of injecting preperfusion and paraformaldehyde solutions into the 
left ventricle, with a cut in the right atrium pre-injection.
  My biggest problem, as I recently tried perfusing a P9 mouse, is that the 
heart is so small that I have yet to successfully place the needle in the 
left ventricle and inject without going through and getting little to no 
perfusion.  That and accessing the right atrium to cut it and allow outflow.

  The heart is quite small.
  Any suggestions?

Thanks,
  Randa Perkins



Randa M. Perkins
Research Assistant
Florida State University
Tallahassee, FL

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