[Histonet] HELICOBACTER CONTROLS

[Rebecca Barnhart]

The reason we are using e coli for a helicobacter positive control is
just to show the stain is working.  If it is positive for e coli it will
be positive for helicobacter.  Our pathologist does know the difference
between helicobacter and e coli, he is the one that has decided to get a
positive control this way.  We do not do IHC stains, just a geimsa
stain.  

>>> "Nick Kirk" <nick.kirk3 <@t> btopenworld.com> 11/11 2:58 PM >>>

Um, surely the whole point of a Helicobacter control, is that it has
Helicobacter in it, not E. coli?
Helicobacter and E. coli look very different for one thing, so by
having a
control with E. coli in it wouldn't give you the necessary reference
point
as it were, to the appearance of Helicobacter positivity.
An inexperienced person might miss a positive if they don't see the
real
thing.
If you're only interested in demonstrating that there's bacteria
present,
why not use the same control that you use for a gram stain?
Personally I would reject any stained slide done in my lab for
Helicobacter,
that wasn't accompanied by a Helicobacter positive control.

Nick Kirk
Histopathology
Hinchingbrooke Hospital
Huntingdon
England


-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu 
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Rebecca
Barnhart
Sent: 11 November 2003 17:29
To: histonet <@t> lists.utsouthwestern.edu 
Subject: Re: [Histonet] HELICOBACTER CONTROLS


We were having trouble finding a positive control also.  So we had our
micro department grow e-coli.  We then smeared it on a piece of colon,
processed, embedded, cut, stained and picked the best section.

Becky

>>> Tasha Bourm <tbourm <@t> olympicmedical.org> 11/11 12:00 PM >>>
Does anyone have a good source for helicobacter QC cut slides or
blocks?

Tasha R. Bourm
Olympic Medical Center
Histology Department
939 Caroline Street
Port Angeles, WA 98362
tbourm <@t> olympicmedical.org 


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