[Histonet] RE: CD21

[GUTIERREZ, JUAN]

Pardon the interruption, but what dilution are you using on the Benchmark?
 
Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Healthcare
 

	-----Original Message----- 
	From: Drew Sally A. [mailto:sa.drew <@t> hosp.wisc.edu] 
	Sent: Tue 11/11/2003 8:18 AM 
	To: Histonet 
	Cc: 
	Subject: [Histonet] RE: CD21
	
	

	We do not use HIER, we use Ventana's protease 1 for 8" with
	BioCare Medical's CD21...have you tried reducing the protease time
	or diluting the  antibody more?  Maybe a different clone would
	please them more...
	
	Sally Ann Drew-MT(ASCP)
	Univ. of Wisconsin Hospital & Clinics
	IHC/ISH Laboratory
	600 Highland Ave. VAH-DM223
	Madison, WI 53792-2472
	608-265-6596
	sa.drew <@t> hosp.wisc.edu
	
	
	-----Original Message-----
	From: Mcleod [mailto:hmcleod <@t> chempath.uct.ac.za]
	Sent: Tuesday, November 11, 2003 8:03 AM
	To: histonet <@t> lists.utsouthwestern.edu
	Subject: [Histonet] CD21
	
	
	Dear All
	We use the Dakocytomation (M0784) CD21 with 10mins Protease AR on the
	Ventana Nexes - IVIEW DAB.  We get epthelial staining as well as the
	expected pattern of staining.  The spec sheet states that this does
	occur.  The problem is our path's are not happy.  Is anybody using
	HIER.  If yes what dilutions are used?
	
	Thanks for all answers.
	Heather
	
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