FW: [Histonet] PECAM staining on FFPE sections

[Stylli, Stanley]

A lttle clarification....the antibody is a Pharmingen antibody available through Becton Dickinson

-----Original Message-----
From: Stylli, Stanley 
Sent: Monday, 10 November 2003 7:50
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] PECAM staining on FFPE sections
Importance: High


Dear All,
I am was wondering if anyone has used the following Becton Dickinson antibody Cat No. 550310-Rat CD31 Pure MAB (TLD-3A12) on FFPE rat tissue sections ? What antigen retrieval techniques have you used ?
The antibody requires the tissue to be zinc fixed but was wondering if anyone has had any luck on formalin fixed sections ? I have had luck with rat kidney but not much with rat brain.
Has anyone used another PECAM antibody that has worked well on FFPE rat brain ?
Thankyou in advance.
Stan Stylli
 

Stan Stylli 
Department of Surgery, 
5th Floor Clinical Sciences Building 
Royal Melbourne Hospital 
University of Melbourne 
Parkville, Australia, 3052. 
Tel: 61-3-93427616 
Fax:61-3-9347-7695 

 

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