[Histonet] DNA extraction from wax sections

Patti Loykasek ploykasek <@t> phenopath.com
Thu Nov 6 16:15:06 CST 2003


There is a product called Gene Releaser from Bioventures, Inc     that works
well for extracting DNA from cut sections.


Patti

> I have a protocol for melting tissue and cutting it up and then extracting
> DNA from the unembedded tissue.  Is this what you want.
> Patsy
> 
> -----Original Message-----
> From: histonet-admin <@t> lists.utsouthwestern.edu
> [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Michele
> Ellender
> Sent: Thursday, November 06, 2003 8:55 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] DNA extraction from wax sections
> 
> Dear all,
> Does anyone out there have a protocol to extract DNA from sections of
> formalin fixed wax embedded mouse tissues?
> I've been asked to microdissect GI tumours from wax sections and extract the
> DNA for use in PCR techniques.
> Hope someone can help.
> Many thanks,
> Michele
> 
> Dr Michele Ellender
> Radiation Effects Department,
> NRPB, Chilton, Didcot,
> Oxon. OX11 0RQ.  UK
> 
> michele.ellender <@t> nrpb.org
> 
> 
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