[Histonet] DNA extraction from wax sections

[Patti Loykasek]

There is a product called Gene Releaser from Bioventures, Inc     that works
well for extracting DNA from cut sections.


Patti

> I have a protocol for melting tissue and cutting it up and then extracting
> DNA from the unembedded tissue.  Is this what you want.
> Patsy
> 
> -----Original Message-----
> From: histonet-admin <@t> lists.utsouthwestern.edu
> [mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Michele
> Ellender
> Sent: Thursday, November 06, 2003 8:55 AM
> To: 'histonet <@t> lists.utsouthwestern.edu'
> Subject: [Histonet] DNA extraction from wax sections
> 
> Dear all,
> Does anyone out there have a protocol to extract DNA from sections of
> formalin fixed wax embedded mouse tissues?
> I've been asked to microdissect GI tumours from wax sections and extract the
> DNA for use in PCR techniques.
> Hope someone can help.
> Many thanks,
> Michele
> 
> Dr Michele Ellender
> Radiation Effects Department,
> NRPB, Chilton, Didcot,
> Oxon. OX11 0RQ.  UK
> 
> michele.ellender <@t> nrpb.org
> 
> 
> 
> This e-mail transmission is strictly confidential and intended
> solely for the person or organisation to whom it is
> addressed.  It may contain privileged and confidential
> information and if you are not the intended recipient,
> please do not copy, distribute or take any action in
> reliance on it.  If you have received this e-mail in error,
> please notify the sender as soon as possible and delete
> the message.
> 
> Please note that NRPB monitors incoming and outgoing
> e-mail for compliance with its Acceptable Use Policy. This
> will include scanning incoming e-mails to detect viruses
> and key-words and may in some circumstances result in
> the manual monitoring of the content of messages.
> 
> National Radiological Protection Board
> E-mail: nrpb <@t> nrpb.org
> Web site: www.nrpb.org
> --------------------------------------------
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>