[Histonet] CD4/CD8

[Amos Brooks]

Manuelle,
         I would certainly recommend using Envision (or some non-biotin 
based detection system). Both antibodies work best using high pH retrieval 
which tends to necessitate Envision.
         I can't speak for Dako CD4 as we use Novocastra but we get much 
better results doing the peroxidase block prior to antigen retrieval. The 
H2O2 interferes with the signal. It sounds like a pain but the results are 
clearly better.
good luck,
Amos Brooks

At 11:35 AM 11/6/03, you wrote:
>Message: 19
>Reply-To: "Manuelle De Bock" <manuelle.debock <@t> UGent.be>
>From: "Manuelle De Bock" <manuelle.debock <@t> UGent.be>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Date: Thu, 6 Nov 2003 11:36:03 +0100
>Subject: [Histonet] CD 4/CD 8 staining of frozen stomach sections of mice
>
>This is a multi-part message in MIME format.
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>Hello everyone,
>
>I am trying (for a long time allready) to produce a good CD4/CD8 =
>staining of frozen stomach tissue (mice). We use the Dako rat anti-mouse =
>CD4 antibody but always encounter a large amound of background  after =
>staining (it seems like there is a lot of cross reactivity with the =
>glandular epithelium). Can anyone help me to solve this problem ?
>Thanx !
>
>
>Manuelle De Bock, dierenarts (DVM)
>Laboratory of Veterinary Pathology=20
>Department of Pathology, Bacteriology and Avian Diseases
>Faculty of Veterinary Medicine
>Ghent University
>Salisburylaan 133
>B9820 Merelbeke - Belgium
>Tel 0032(0)9 264 7745
>Fax 0032(0)9 264 7789