[Histonet] ethopropazine/acetylcholinesterase histochemistry

[sebres]

Thanks much!    I spoke on the phone with Sigma before posting my question &
was told by them that they no longer carry the ethopropazine, nor did they
have any advice on a substitute for me.  Based on Ronnie's experience it
doesn't sound worth sinking a lot of $ into this, but as luck would have it,
my husband, a patchclamper, has some  iso-OMPA that I can use.  Histonet is
the greatest!--one of our posters at Society for Neuroscience this weekend
is on teaching histology in a univ psych dept (in the "teaching
neuroscience" posters) & I hope you folks don't mind if I include a little
PR for Histonet on it!  gratefully,   Susan
----- Original Message ----- 
From: "Smith, Allen" <asmith <@t> mail.barry.edu>
To: "sebres" <sebres <@t> comcast.net>
Cc: <histonet <@t> lists.utsouthwestern.edu>
Sent: Thursday, November 06, 2003 11:26 AM
Subject: RE: [Histonet] ethopropazine/acetylcholinesterase histochemistry


Back in the dark ages, we used diisopropylfluorophosphate as a
cholinesterase inhibitor.  It's very effective (and very expensive), but not
very specific: it also inhibits trypsin, thrombin, and plasmin.
Diisopropylfluorophosphate is still available from SIGMA (1-800-325-3010),
cat # D 0879 @ 227.65 / g.
Ethopropazine is still in the current SIGMA catalog, cat # E 2880 @ 16.60 /
5 g.

-----Original Message-----
From: sebres [mailto:sebres <@t> comcast.net]
Sent: Wednesday, November 05, 2003 6:36 PM
To: Histonet (E-mail)
Subject: Re: [Histonet] ethopropazine/acetylcholinesterase histochemistry


Well, I've already dug a little deeper & come up with part of the answer,
but am still in need of advice:  I understand better now that it is
specifically butyrylcholinesterase that ethopropazine is supposed to
inhibit.  I've found some evidence that ethephon is considered a specific
butyrylcholinesterase inhibitor, & this is actually available for purchase
from agricultural supply companies.  Any thoughts out there on whether this
sounds like a viable solution?   Many thanks,  Susan
----- Original Message ----- 
From: "sebres" <sebres <@t> comcast.net>
To: "Histonet (E-mail)" <histonet <@t> lists.utsouthwestern.edu>
Sent: Wednesday, November 05, 2003 6:03 PM
Subject: [Histonet] ethopropazine/acetylcholinesterase histochemistry


> I'm teaching a neurohistology class in a research university, mainly
> Nissl staining, immunohistochemisty & in situ hybridization
> histochemistry on
rat
> brain sections.  I thought I'd add to the mix a good old fashioned
> enzyme histochemistry assay, such as the elegant acetylcholinesterase
> method described in Paxinos & Watson's Rat Brain atlas, which sounds
> refreshingly simple.  But, to my shock, ethopropazine, since it is now
> used medicinally (Parsitan), seems to no longer be available except by
> prescription!  If I understand this correctly, the main purpose of
> this reagent in this assay
is
> as a cholinesterase inhibitor, in which case I'm wondering whether it
might
> work to substitute either physostigmine, or possibly haloperidol, both
> of which I already have in hand?  My students and I would all be extremely
> grateful for any advice about this!     Susan Bachus, George Mason
> University
>
>
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> Histonet <@t> lists.utsouthwestern.edu
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