[Histonet] Distinguishing monocytes from granulocytes

Mark Frei mfrei <@t> sial.com
Wed Nov 5 13:07:56 CST 2003


Have you thought of cytochemistry?
Chloroacetate esterase and naphthyl acetate esterase are two enzymatic 
assays that will easily and cheaply differentiate the mentioned cell 
types.




Ramesh Subrahmanyam <ramesh <@t> brandeis.edu>
Sent by: histonet-admin <@t> lists.utsouthwestern.edu
11/05/2003 11:46 AM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Distinguishing monocytes from granulocytes


Hello Histonetters,
I am trying to distinguish immature monocytes from immature granulocytes 
in Balb/c mice. Can anyone suggest antibodies for FACS or 
immunohistochemistry or RT-PCR markers that would help in this? 
Suggestions on other methods would be welcome too.
Thanks in advance,

Ramesh
Brandeis University
Massachusetts


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