[Histonet] Not all tissue staining...

[T. Truscott]

Karen, Ventana can be great help, but you can also check that all your 
reagent containers are clean and that LCS didn't get poured into the wrong 
one. Good Luck Tom Truscott USDA-ARS Pullman, WA


>From: "Laurie Colbert" <laurie.colbert <@t> huntingtonhospital.com>
>To: "Bauer, Karen" <Bauer.Karen <@t> mayo.edu>,"Histonet (E-mail)" 
><histonet <@t> lists.utsouthwestern.edu>
>Subject: RE: [Histonet] Not all tissue staining...
>Date: Wed, 29 Oct 2003 10:47:26 -0800
>
>Karen,
>
>We have four BenchMarks and and a NexES special stainer and whenever we 
>have a problem we call Ventana's customer service.  If they can't 
>troubleshoot over the phone, they are very willing to send a service tech 
>out.
>
>-----Original Message-----
>From: Bauer, Karen [mailto:Bauer.Karen <@t> mayo.edu]
>Sent: Wednesday, October 29, 2003 10:25 AM
>To: Histonet (E-mail)
>Subject: [Histonet] Not all tissue staining...
>
>
>Hello to all,
>
>There are times when a stained IP slide will have only half of the tissue
>staining positively and not other parts of the tissue, but has the same
>cellularity of the part that is staining. (Did that make sense?)  Same
>tissue cells, but some stain and some do not.  When the Pathologist asks 
>why
>this happens, I can only come up with possible vortex mixing problems or
>that the paraffin did not come out completely.  We've recently switched to
>the BenchMark and this has only happened a few times.  I've checked the
>vortex mixers, and all of the heating pads are working properly.
>
>Does anyone have any suggestions?
>
>Thanks in advance.
>
>Karen L. Bauer HT(ASCP)
>Histology Department
>Luther Hospital
>715-838-3205
>
>
>
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