[Histonet] collagen I staining

[Patsy Ruegg]

I had good collagen I staining with antibody from U of Iowa Hybridoma Bank 
with Pepsin digestion on formic acid decalcified rabbit bones.
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg <@t> msn.com
wk email pruegg <@t> colobio.com

This email is confidential and intended solely for the use of the Person(s) 
('the intended recipient') to whom it was addressed. Any views or opinions 
presented are solely those of the author. It may contain information that is 
privileged & confidential within the meaning of applicable law. Accordingly 
any dissemination, distribution, copying, or other use of this message, or 
any of its contents, by any person other than the intended recipient may 
constitute a breach of civil or criminal law and is strictly prohibited. If 
you are NOT the intended recipient please contact the sender and dispose of 
this e-mail as soon as possible.





----Original Message Follows----
From: "Mass Histology Service" <jstaruk <@t> masshistology.com>
To: "Rita Angel" <RITA.ANGEL <@t> UC.EDU>,<histonet <@t> pathology.swmed.edu>
Subject: RE: [Histonet] collagen I staining
Date: Fri, 31 Oct 2003 14:50:30 -0500

We've had good luck with Calbiochem's anti-collagen I (Cat. # CP17L), 1:50
following pronase pre-treatment on rabbit bones.

Jim

____________________
James Staruk, HT(ASCP)
Mass Histology Service
A division of DVMLab, inc.
www.masshistology.com


-----Original Message-----
From: histonet-admin <@t> lists.utsouthwestern.edu
[mailto:histonet-admin <@t> lists.utsouthwestern.edu]On Behalf Of Rita Angel
Sent: Friday, October 31, 2003 2:23 PM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] collagen I staining


Hello Histonetters,

Someone in our lab has been trying to stain for collagen I antibody in
rabbit tissue. She's tried the antibody from several different companies
and has used different antigen retrievals, but has been unable to get any
positive staining. The only staining she's gotten is background. These are
paraffin sections. The companies she bought the antibodies from has not
been helpful. She's stained with Collagen type II & III and had no trouble
with these. Any suggestions would be helpful.

Thank you,
Rita Angel, HT
University of Cincinnati


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_________________________________________________________________
Add MSN 8 Internet Software to your current Internet access and enjoy 
patented spam control and more.  Get two months FREE!     
http://join.msn.com/?page=dept/byoa