[Histonet] open processors

[Shelkowsky, Ben]

When we used to use the open processor, many , many years ago, we used a Tissue Tek vacuum infiltrator for 15 min. before we started embedding.  1. We removed the cassettes from the Technicon and transferred them into the vaccum apparatus. We turned on the vaccuum for 15 min. You can try it first on an experimental basis to see if it makes a difference. At the time we used it we felt we got better infiltration, in general of all the tissues. Have a Happy  New Year!

	-----Original Message----- 
	From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Ford Royer 
	Sent: Mon 12/29/2003 7:09 AM 
	To: Bryan Hewlett 
	Cc: histonet <@t> lists.utsouthwestern.edu; JCarpenter764 <@t> aol.com 
	Subject: Re: [Histonet] open processors
	
	

	Vacuum also removes any trapped air bubbles on the cellular level.
	
	~ Ford
	Ford M. Royer, MT(ASCP)
	Analytical Instrumnets, llc
	Minneapolis, MN
	
	
	Bryan Hewlett wrote:
	
	>Vacuum on an open processor is used only during infiltration with molten
	>wax, it has no other function such as moving fluids which occurs in a closed
	>type processor. Vacuum infiltration with molten wax dramatically reduces the
	>time (and number of wax changes) necessary to remove the clearing solvent
	>and hence reduces the time in hot wax, a good thing.
	>
	>Bryan
	>
	>
	>----- Original Message -----
	>From: <JCarpenter764 <@t> aol.com>
	>To: <histonet <@t> lists.utsouthwestern.edu>
	>Sent: Sunday, December 28, 2003 10:38 AM
	>Subject: [Histonet] open processors
	>
	>
	> 
	>
	>>Can someone tell me the function of the vacuum on an open processor.  I
	>>   
	>>
	>don't
	> 
	>
	>>understand what purpose it serves or its function.   thanks
	>>_______________________________________________
	>>Histonet mailing list
	>>Histonet <@t> lists.utsouthwestern.edu
	>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
	>>
	>>   
	>>
	>
	>
	>
	>_______________________________________________
	>Histonet mailing list
	>Histonet <@t> lists.utsouthwestern.edu
	>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
	>
	> 
	>
	_______________________________________________
	Histonet mailing list
	Histonet <@t> lists.utsouthwestern.edu
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
	

Confidentiality Notice:
This is a transmission from Community Hospital of the Monterey Peninsula.  This message and any attached documents may be confidential and contain information protected by state and federal medical privacy statutes.  They are intended only for the use of the addressee.  If you are not the intended recipient, any disclosure, copying, or distribution of this information is strictly prohibited.  If you received this transmission in error, please accept our apologies and notify the sender.

Thank you.