[Histonet] open processors
Ben.Shelkowsky <@t> chomp.org
Mon Dec 29 09:47:14 CST 2003
When we used to use the open processor, many , many years ago, we used a Tissue Tek vacuum infiltrator for 15 min. before we started embedding. 1. We removed the cassettes from the Technicon and transferred them into the vaccum apparatus. We turned on the vaccuum for 15 min. You can try it first on an experimental basis to see if it makes a difference. At the time we used it we felt we got better infiltration, in general of all the tissues. Have a Happy New Year!
From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Ford Royer
Sent: Mon 12/29/2003 7:09 AM
To: Bryan Hewlett
Cc: histonet <@t> lists.utsouthwestern.edu; JCarpenter764 <@t> aol.com
Subject: Re: [Histonet] open processors
Vacuum also removes any trapped air bubbles on the cellular level.
Ford M. Royer, MT(ASCP)
Analytical Instrumnets, llc
Bryan Hewlett wrote:
>Vacuum on an open processor is used only during infiltration with molten
>wax, it has no other function such as moving fluids which occurs in a closed
>type processor. Vacuum infiltration with molten wax dramatically reduces the
>time (and number of wax changes) necessary to remove the clearing solvent
>and hence reduces the time in hot wax, a good thing.
>----- Original Message -----
>From: <JCarpenter764 <@t> aol.com>
>To: <histonet <@t> lists.utsouthwestern.edu>
>Sent: Sunday, December 28, 2003 10:38 AM
>Subject: [Histonet] open processors
>>Can someone tell me the function of the vacuum on an open processor. I
>>understand what purpose it serves or its function. thanks
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>>Histonet <@t> lists.utsouthwestern.edu
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