[Histonet] proteins in vesicles staining

Fri Dec 19 05:08:20 CST 2003

Hello everybody!!!

I want to stain in IHC a protein (guanylin) which is supposed to be in
intra-cellular vesicles in the epithelium. Could somebody let me know (1)
if there is a protocol (and which one) to premeabilize these vesicles to
allow the AB to fix on the protein inside? or (2) if something better does
exist?

Thank you very much.
Merry Christmas.

Anne-Sophie

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Dr Anne-Sophie Martinez
School of Biology
University of St. Andrews
Bute Medical Buildings
St. Andrews
Fife, KY16 9TS
UK.

Tel + 44 (0) 1334 463546
Fax + 44 (0) 1334 463600