[Histonet] gomori methenamin silver

Gudrun Lang gudrun.lang <@t> aon.at
Wed Dec 17 08:16:37 CST 2003

Dear histonetters,
I need help of those, who are familiar to Gomori Methenamin Silver staining.
I have troubles to convince my colleages about the advantages of using a stock meth-silver-solution. (instead of fresh making each time)
stock:    1:40 10%Silvernitrate : 3% Hexamethenamin (stable for few months); 
working:  1:10 Borax : stock
My colleages think, that the stock solution soon becomes instable. And that there is a kind of sediment at the bottom of the bottle (more concentration of silver).

Can anyone tell me about bad experience with older stock solutions? What happens, when the stock solution is older than two (or three,..) months? Is the result of the stain weaker or darker?

Question 2:
What is the the function of Hexamethenamin? I know it causes the high pH, but does it make any compound with the silvernitrate?

Any help is welcome
Best wishes from Austria

Gudrun Lang
Akh Linz, Austria
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