[Histonet] Re : Kappa and Lambda

Anissa Choi anissachoi <@t> hotmail.com
Tue Dec 16 21:57:21 CST 2003


Does anyone pressure cook the paraffin sections before  immunostaining for 
Kappa and Lambda?
Our regular protocols : no pretreatment for B5, Protease for buffered 
formalin. I have found that
there are a lot more plasma cells get stained after pressure cooking but 
backround is heavy though.

Thanks in advance for your input.

Anissa Choi
Burnaby Hospital
Burnaby, B.C.
Canada

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