[Histonet] mouse embryo section tears
rashmi <@t> neuro.duke.edu
Mon Dec 15 09:43:59 CST 2003
I am sectioning paraffin embedded mouse embryos (fixed in 4%
paraformaldehyde) at 7 microns. The sections cut and ribbon fine but after
i dry them on the slide, tears appear in the liver. These tears are not
present when I first check the sections after lifting them from the water
bath (temperature 37 C) onto the slide. I think the tears may result from
differential drying of the various tissues, and I am looking for a way to
Thanks a lot for your advice!
Rashmi Chandra, Ph.D.
Dept. of Neurobiology
Duke University Medical Center
Durham, NC 27710
Tel: (919) 681-5449
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