[Histonet] RE: IHC on cells on coverslips

Tony Henwood AnthonyH <@t> chw.edu.au
Sun Dec 14 21:34:39 CST 2003


Yes, granted S100 is alcohol soluble, but for melanoma confirmation, one can
use HMB-45 or Melan-A.
Remember, sometimes one may only have one or two cell smears available in
order to confirm a diagnosis (eg no material in the cell block) so one must
resort to one of the available smears. In this instance, collecting evidence
and expertise is important. We need to appreciate the usefulness and
shortcommings of the antibodies we use.

It does seem that there are more antibodies that work on ethanol fixed
smears than those that do not.

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318


-----Original Message-----
From: Nader, Alexander [mailto:alexander.nader <@t> wgkk.sozvers.at]
Sent: Friday, 12 December 2003 11:36 PM
To: Histology Net List Server (E-Mail)
Subject: [Histonet] RE: IHC on cells on coverslips

> "Not really beneficial to many antigens"? I could list at least 50
> antibodies that work on ethanol fixed smears. Most of these 
> are in routine
> use.
> eg: AE1AE3, Cam5.2, 5D3, Vimentin, Desmin, GFAP, PSAP, PSA, CEA, EMA,
> Skeletal muscle myosin, Actin,etc etc.

S100-Antigen, GCFBP and some other ab's "leach out" with ethanol fixation,
even fixation of trephines with Schaffer's solution (ethanol/formalin
mixture) shows this unwanted and rather nasty effect.

Alexander Nader MD
Vienna, Austria

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